Determination of the targeted carbendazim metabolites in zebrafish water tank by liquid chromatography coupled to high-resolution mass spectrometry

Carbendazim, a common pesticide, has shown signs of causing cancer, infertility and toxicity to organisms. Due its intense use, this fungicide become persistent compound in the environment, raising importance better understanding behaviour, metabolic pathways effects on Zebrafish is considered an excellent animal model, being able rapidly absorb compounds water, mimicking what occurs aquatic environment. Therefore, aim work was evaluate carbendazim metabolites zebrafish water tank using liquid chromatography coupled high-resolution mass spectrometer (LC-HRMS) highlight analytical targets order monitor exposure environments. For purpose, treatment, negative stability groups were defined. In addition, samples collected from tanks analyses carried out by LC-HRMS. The results allowed putative annotation 7 target metabolites. This study applied analysis for evaluation as promising approach, since it much cleaner matrix than usual biological matrices. These can ensure detectability further strengthen confidence monitoring To best our knowledge, there no one that evaluated produced zebrafish, neither animal’s body nor tank. first report generation zebrafish.